Dioxygen reactivity ofmeso-hydroxylated hemes: Intermediates in heme degradation process catalyzed by heme oxygenase
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چکیده
منابع مشابه
Features of the reaction of heme degradation catalyzed by the reconstituted microsomal heme oxygenase system.
The heme oxygenase system was reconstituted from a heme oxygenase preparation highly purified from pig spleen microsomes and a partially purified NADPHcytochrome c reductase from pig liver microsomes. In the reconstituted heme oxygenase reaction, the relationship between the rate of heme degradation and the heme concentration was sigmoidal, and the heme concentration which gave the half-maximum...
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Hmu O, a heme degradation enzyme in the pathogen Corynebacterium diphtheriae, catalyzes the oxygen-dependent conversion of hemin to biliverdin, carbon monoxide, and free iron. A bacterial expression system using a synthetic gene coding for the 215-amino acid, full-length Hmu O has been constructed. Expressed at very high levels in Escherichia coli BL21, the enzyme binds hemin stoichiometrically...
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Heme oxygenase (HO), in conjunction with biliverdin reductase, degrades heme to carbon monoxide, ferrous iron and bilirubin (BR); the latter is a potent antioxidant. The induced isoform HO-1 has evoked intense research interest, especially because it manifests anti-inflammatory and anti-apoptotic effects relieving acute cell stress. The mechanisms by which HO mediates the described effects are ...
متن کاملMicrosomal Heme Oxygenase
This study characterizes microsomal heme oxygenase, a previously undescribed enzyme which catalyzes the oxidation of heme at the cr-methene bridge to form biliverdin. This step is then coupled with soluble NADPH-dependent biliverdin reductase to form bilirubin; microsomal heme oxygenase is rate-limiting in this pathway. By all analytical criteria, the product of this reaction is bilirubin. Most...
متن کاملInduction of Heme Oxygenase -1 By Lipocalin 2 Mediated By Nf-Kb Transcription Factor
Purpose: Effect of lipocalin 2 on the expression of heme oxygenase I , II and NF-kB transcription factor was the purpose of this survey. Materials and Methods: Lcn2 was cloned to pcDNA3.1 plasmid by using genetic engineering method. The recombinant vector was transfected to CHO and HEK293T to establish stable cell expressing lipocalin 2. The presence of lipocalin 2 gene in these cells was confi...
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ژورنال
عنوان ژورنال: Journal of Chemical Sciences
سال: 2006
ISSN: 0253-4134,0973-7103
DOI: 10.1007/bf02703943